25 de noviembre del 2020: Advanced Waves Sensors S.L. (AWSensors) ha sido beneficiaria del Fondo Europeo de Desarrollo Regional cuyo objetivo es mejorar la competitividad de las Pymes y gracias al cual ha puesto en marcha un Plan de Internacionalización con el objetivo de mejorar su posicionamiento competitivo en el exterior durante el año 2020. Para ello ha contado con el apoyo del Programa XPANDE DIGITAL 2019 de la Cámara de Comercio de Valencia.
October 15th 2020: AWSensors is pleased to invite you to take a look to its Technology Note entitled “QCMD in Lipid Research”.
QCMD is a label-free surface-analytical technique based on a quartz resonator excited to oscillate at its resonance frequency on one or more overtones. Resonators can have various coatings: gold (Au), silica (SiO2), titania (TiO2), etc. It works in aqueous media or organic solvents and is therefore widely used for studying solid/liquid interfaces. At each overtone, QCMD measures changes in the resonance frequency and energy dissipation due to the processes occurring at the resonator surface. Examples of such processes include formation of a film or changes in the geometrical or physical properties of the film.
The key feature that makes QCMD useful in lipid research is its ability to distinguish between different geometries and topologies of lipidic assemblies at interfaces, for example, homogenous solid-supported bilayers or monolayers vs. adsorbed liposomes or other structures (such as cubosomes) without relying on fluorescent or deuterated labels but by relying on the combination of the frequency and dissipation.
Lipid-related QCMD work can be grouped into several topics, with a total of more than a thousand publications:
• Studies focusing on the interactions between lipids and surfaces.
• Studies focusing on the properties of the lipids, such as their phase behavior, adsorbed liposome deformation, etc.
• Studies examining interactions between lipids and membrane-binding proteins, peptides or viruses. Particularly interesting is that QCMD offers a way to study clustering of membrane-bound proteins.
• Studies focusing on the interactions of lipids with polymers or with nanoparticles.
Continue reading by downloading the full Technology Note (below) …
You can download the full Note in pdf format through this link. A list of our Technology Notes can be found on our Technology Web Page.
Authors: Pierre Lemaire, Thomas Dargon, Daniel Alves Dalla Corte, Ozlem Sel, Hubert Perrot, and Jean-Marie Tarascon
Journal: Anal. Chem., 2020
Authors: Gerardo Prieto, Vicente Domínguez-Arca, Rui R. Costa, Ana M. Carvalho, Pablo Taboada, Rui L. Reis, Iva Pashkuleva
Journal: Research Square, 2020
Authors: Román Fernández; María Calero; Ilya Reviakine; José Vicente García; María Isabel Rocha-Gaso; Antonio Arnau; Yolanda Jiménez
Journal: IEEE Sensors Journal, 2020
September 15th 2020: AWSensors is pleased to invite you to take a look to its new Technology Note entitled “Tracking Recovery Technology Note”.
Use of the AWSensors X1 Instrument Tracking Recover feature to monitor overtones frequency and dissipation shifts of air-to-liquid medium exchanges onto 5 MHz QCM sensors.
The Tracking Recovery feature included in AWSensors X1 platform allows the user to monitor large and fast frequency shifts in QCM (Quartz Crystal Microbalance) admittance spectrum. These sudden modifications in the sensor response are common is some applications where dramatical changes in the viscoelastic properties of the sensor surrounding medium take place.
This technical note illustrates the utility of tracking recovery feature to characterize an air-to-liquid medium exchange. According to Kanazawa and Gordon theory predictions [1], a complex frequency shift is expected in the sensor electromechanical response when the semi-infinite medium placed over the QCM’s top electrode is replaced by other semi-infinite medium. This shift will depend on the viscosity and density properties of the final medium. Following, Kanazawa-Gordon equation is presented for both the frequency (Eq. 1) and the half-bandwidth (Eq. 2) shifts.
Continue reading by downloading the full Technology Note (below) …
You can download the full Note in pdf format through this link. A list of our Technology Notes can be found on our Technology Web Page.
July 3rd 2020: AWSensors is pleased to invite you to take a look to the Biosensor Application Note entitled “Acoustic Biosensor“.
An immunosensor application for determination of carbaryl pesticide was developed by using AWS A20 research platform and AWS F20 Fluidic System. Carbaryl was chosen as the model analyte. Two kinds of acoustic sensors were employed: AWS HFF-QCM sensors (50 MHz and 100 MHz) and Love-SAW sensors with appropriate cells. The AWS A20 platform allowed monitoring phase-shift changes at constant frequency as a function of the sensor surface mass changes.
Sensor functionalization: Carbaryl hapten conjugate was covalently immobilized by means of Self Assembled Monolayer (SAM).
Immunoassay format: The chosen competitive immunoassay was a binding-inhibition test based on conjugate-coated format. Carbaryl analyte competes against the immobilized hapten-conjugate for Monoclonal Antibodies.
Carbaryl detection: Samples were injected onto the sensors’ surfaces. AWS software allowed controlling sample injection and fluidics. Furthermore, the employed platform allowed performing the measurements at a constant temperature of 25°C ± 0.05°C.
Since analyte inhibits antibody binding to its respective immobilized conjugates, increasing concentrations of analyte are detected by a change in the increment of the phase-shift of the sensor. The following figures present a representative assay cycle selected from a continuous monitoring in a carbaryl determination, for 100 MHz HFF QCM and 120MHz Love Wave Sensors.
Continue reading by downloading the full Application Note (below) …
You can download the full Application Note in pdf file from this link or download it from our Applications Web Page where you can find this and the rest of our Application and Technology Notes.
Authors: Martin Zalazar; Gabriel Muñoz; Rodrigo M Torres
Journal: Investigative Ophthalmology & Visual Science, 2020
Authors: Jeffrey E. Chen, Qifeng Wang, Kenneth R. Shull, Jeffrey J. Richards
Journal: Journal of Colloid and Interface Science, 2020
