AWSensors showed its work on carbaryl pesticide during the 4th International Conference on Biosensing Technology held in Lisbon, Portugal, last month. Attendees were very interested in how AWS platform provides ELISA limit of detection. Therefore, we would like to explain and share on line the work entitled “Further sensitivity enhancement of HFF-QCM immunosensors for pesticides”.
AWSensors have successfully developed a High Fundamental Frequency Quartz Crystal Microbalance that improves sensitivity of QCM biosensors. Taking advantage of this technology, AWS developed and tested a renewed highly sensitive HFF piezoelectric immunosensor for its use in pesticide detection, using insecticide carbaryl as a model analyte. To this purpose, 100 MHz quartz crystals were used as the transducer elements of the biosensor and a monoclonal antibody-based
competitive immunoassay was integrated as the sensing specific bio-recognition event. The biosensing interface was improved by employing mixed self-assembled monolayers of alkane thiols as intermediate layers for surface functionalization. This approach allowed the covalent attachment of the assay conjugate on the gold electrode surface in a more orderly and stable way than with simple SAMs. Moreover, a very low concentration of LIB-CNH45 monoclonal antibody was used for the competitive immunoassays. The sensitivity enhancement achieved turns the HFF-AWS pesticide immunosensor into one of the most sensitive analytical tools for this kind of low molecular compounds. Don’t lose the opportunity to take advantage of this innovation. AWSensors offers its new technology at the best price.
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