Publication on AWSensors technology

Multifunctional Surfaces for Improving Soft Tissue Integration

Authors: Adriana Vilaça, Rui M. A. Domingues, Hanna Tiainen, Bárbara B. Mendes, Alejandro Barrantes, Rui L. Reis, Manuela E. Gomes, Manuel Gomez‐Florit.

Journal: Advanced Healthcare Materials, 2021

Antibodies

Antibody Detection with Quartz Crystal Microbalance

May 10th 2021: AWSensors offers acoustic sensors for antibody detection. Acoustic biosensor-based immunosensing with  High-Fundamental Frequency Quartz Crystal Microbalance (HFF-QCM) and Love-SAW sensors offers several advantages over Enzyme Linked Immuno Assay (ELISA) or Surface Plasmon Resonance (SPR).

Acoustic sensors antibody detection advantages over ELISA

  • Sensitivity approximately as a standard ELISA or better.
  • Acoustic sensors offer label-free detection of the antibody-antigen binding.
  • Full automation.
  • Pre-calibrated (the user does not need to run the standards. More samples can be run. Reduced cost per sample).
  • Speed.
  • Quantitative real-time /in-situ monitoring.
  • Turn-key solution (no need to specialized detection equipment, reader, or spectrophotometer).
  • Reduced complexity, relaxed requirements for trained personnel.
  • Small volumes (~ 10 of ul per sample).
  • Parallelization and multiple analyte detection through sensor arrays.
  • Sensor re-usablity, leading to reduced assay cost.

Acoustic sensors antibody detection advantages over SPR

  • Better sensitivity.
  • QCM also sees solvent.
  • Provides conformational information of the surface film.
  • Miniaturization and integration into portable systems.
  • Easier to develop parallel and multiple analyte assays through sensor arrays.
  • Affordability.

Therefore, one can develop biosensors based on acoustic immunosensors with highly attractive features in order to rapidly detect pathogens like viruses and bacteria [1], and low molecular weigh compounds.

Application Example

Our Biosensor Application Note  is an application example where we describe a sensitive detection of a low molecular weight pesticide carbaryl using competitive immunoassay with hapen-conjugates immobilized on high-fundamental frequency QCMD sensors, SAMs and monoclonal antibodies (MAb) [2, 3]. As a result, we achieved a very sensitive detection of the carbaryl analyte.

Biosensor

 

References

[1] Surface Generated Acoustic Wave Biosensors for the Detection of Pathogens: A Review 

[2] High-frequency phase shift measurement greatly enhances the sensitivity of QCM immunosensors

[3] Love Wave Immunosensor for the Detection of Carbaryl Pesticide  

Publication on AWSensors technology

Exploring the effect of humidity on thermoplastic starch films using the quartz crystal microbalance

Authors: Matthew D. Eaton,  Daniel Domene-López,  Qifeng Wang, Mercedes G. Montalbán,  Ignacio Martin-Gullon, Kenneth R.Shull.

Journal: Carbohydrate Polymers, 2021

Publication on AWSensors technology

Scrutiny of the LiCoO2 Composite Electrode/Electrolyte Interface by Advanced Electrogravimetry and Implications for Aqueous Li-Ion Batteries

Authors: Wanli Gao, Natacha Krins, Christel Laberty-Robert, Hubert Perrot, and Ozlem Sel

Journal: The Journal of Physical Chemistry, 2021

Publication on AWSensors technology

Well-Defined Lignin Model Films from Colloidal Lignin Particles

Authors: Muhammad Farooq , Zou Tao, Juan José Valle-Delgado, Mika Henrikki Sipponen, Maria Morits, Monika Österberg

Journal: Langmuir, 2020

Publication on AWSensors technology

A Fast Method for Monitoring the Shifts in Resonance Frequency and Dissipation of the QCM Sensors of a Monolithic Array in Biosensing Applications

Authors: Román Fernández; María Calero; José Vicente García-Narbón; Ilya Reiviakine; Antonio Arnau; Yolanda Jiménez

Journal: IEEE Sensors Journal, 2021

Publication on AWSensors technology

Correlation between the interfacial ion dynamics and charge storage properties of poly (ortho-phenylenediamine) electrodes exhibiting high cycling stability

Authors: El Mahdi Halim, Rezan Demir-Cakan, Hubert Perrot, Mama El Rhazi, Ozlem Sel.

Journal: HAL, 2020

Publication on AWSensors technology

Two-dimensional adaptive membranes with programmable water and ionic channels

Authors: Daria V. Andreeva, Maxim Trushin, Anna Nikitina, Mariana C. F. Costa, Pavel V. Cherepanov, Matthew Holwill, Siyu Chen, Kou Yang, See Wee Chee, Utkur Mirsaidov, Antonio H. Castro Neto & Kostya S. Novoselov.

Journal: Nature Nanotechnology, 2020

Potentiostat Integration for EQCM

Potentiostat Integration Application Note

March 15th 2021: AWSensors is pleased to invite you to take a look to the its new Application Note on Potentiostat Integration entitled “Potentiostat integration with AWSensors equipment“.

Summary of the Note

Seamless integration of QCMD and electrochemistry in the AWSensors EQCMD systems allows simultaneous measurements of the amount, electrochemical properties, and organization of material at the air/liquid interface accessed through the changes in the resonance frequency and dissipation measured by QCMD and the potential/current relationships measured with an integrated potentiostat or a galvanostat. The capabilities of the integrated AWSensors electrochemical EQCMD systems are illustrated here using the electropolymerization of aniline as an example.

Potentiostat Integration for EQCM

Introduction

Investigation of complex interfacial processes benefit from combinations of complementary surface-analytical techniques that are based on different principles and approach the interface from complementary perspectives. [1,2] In this regard, the electrochemical quartz crystal microbalance with dissipation measurements, or EQCMD, has a venerable history. [3-5] Indeed, one of the incentives for immersing QCMD in liquids in the 1980s was to perform combined EQCMD measurements [6]. The two approaches are complementary in terms of the information they provide about the solid-liquid interface: interfacial mass transfer and structural changes are accessed with QCMD, while electrochemistry is concerned with the interfacial charge transfer and surface potential changes. Of particular interest is the quantitative characterization of electrochemically driven structural or viscoelastic transitions in interfacial layers, e.g., in battery research [7].

To accommodate the needs of researchers working with electrochemical applications in a wide variety of fields, AWSensors developed QCMD instruments with integrated potentiostat/galvanostat control for synchronizing QCMD and electrochemical experiments with appropriate BioLogic potentiostat or galvanostat. To illustrate the functionality, we use a straight-forward aniline polymerization experiment.

Electropolymerization of aniline to form polyaniline (PANI), and its electrochemical properties, have been widely studied, including by electrochemical quartz crystal microbalance (ref. [8-10], and references therein). Here, we go through the necessary steps for setting up an electrochemical experiment with the integrated QCMD/potentiostat combination for following aniline electropolymerization on the gold electrode surface of a QCMD sensor. The gravimetric and electrochemical results are presented, and the gravimetric results are compared with the cyclic voltammetry.

Continue reading by downloading the full Note (below) …


Download Full Note

You can download the full Application Note in pdf file from this link or download it from our Applications Web Page where you can find this and the rest of our Technology Notes.

Other references about the Note

 

Publication on AWSensors technology

Deciphering the Influence of Electrolytes on the Energy Storage Mechanism of Vertically-Oriented Graphene Nanosheet Electrodes by Using Advanced Electrogravimetric Method

Authors: Tao Lé, Gérard Bidan, Florence Billon, Marc Delaunay, Jean-Michel Gérard, Hubert Perrot, Ozlem Sel and David Aradilla

Journal: Nanomaterials, 2020