Journal: Biosensors (2020)

Authors: María Calero, Román Fernández, Pablo García, José Vicente García, María García, Esther Gamero-Sandemetrio, Ilya Reviakine, Antonio Arnau and Yolanda Jiménez.


Integrating acoustic wave sensors into lab-on-a-chip (LoC) devices is a well-known challenge. We address this challenge by designing a microfluidic device housing a monolithic array of 24 high-fundamental frequency quartz crystal microbalance with dissipation (HFF-QCMD) sensors. The device features six 6-L channels of four sensors each for low-volume parallel measurements, a sealing mechanism that provides appropriate pressure control while assuring liquid confinement and maintaining good stability, and provides a mechanical, electrical, and thermal interface with the characterization electronics. We validate the device by measuring the response of the HFF-QCMD sensors to the air-to-liquid transition, for which the robust Kanazawa–Gordon–Mason theory exists,
and then by studying the adsorption of model bioanalytes (neutravidin and biotinylated albumin). With these experiments, we show how the e ects of the protein–surface interactions propagate within adsorbed protein multilayers, o ering essentially new insight into the design of anity-based bioanalytical sensors.

Link: doi:10.3390/bios10120189