Tag Archive for: biosensors

Scientific publication

High Frequency (100, 150 MHz) Quartz Crystal Microbalance (QCM) Piezoelectric Genosensor for the Determination of the Escherichia coli O157 rfbE Gene

Authors: Kaory Barrientos, María Isabel Rocha, Marisol Jaramillo and Neil Aldrín Vásquez.
Journal: Analytical Letters (2022)

 

Abstract

Escherichia coli O157 (E. coli O157) is responsible for outbreaks of high morbidity in food-borne infections. The development of sensitive, reliable, and selective detection systems is of great importance in food safety. In this work, two high fundamental frequency (HFF) piezoelectric genosensors (100 and 150 MHz) were designed and validated for the rfbE gene detection, which encodes O-antigen in E. coli O157. HFF resonators offer improved sensitivity, small sample volumes, and the possibility of integration into lab-on-a-chip devices, but their sensing capabilities have not yet been fully explored. This HFF-QCM genosensor uses the method of physisorption based on the union between the streptavidin and the biotin to immobilize the genetic bioreceptor on the surface and detect its hybridization with the target sequence. Parameters such as molecular coating, specificity, and variability were tested to enhance its performance. Although both genosensors evaluated are able to determine the target, the 100 MHz device has a higher response to the analyte than the 150 MHz platform. This is the first step in the development of an HFF-QCM genosensor that may be used as a trial test of E. coli O157 in large batches of samples.

 

You may read the full paper here.

The difference the “D” makes in QCMD

AWSensors presents a new Technology Note on the importance of dissipation measuring when working with QCMD: “The difference the “D” makes in QCMD”.

Dissipation

What is dissipation, and why it is useful?

Quartz Crystal Microbalance with Dissipation, or QCMD, is having a tremendous impact on research in the soft and biological interfaces fields because of its versatility and the wealth of information it provides. In this Technology Note, we discuss the origins of dissipation in the different systems studied by QCMD, from complex fluids and polymer films to biomolecular and particle assemblies, and the information dissipation can provide, from characterizing viscoelasticity to studying molecular conformation.

In this Technical Note we discuss the origins of dissipation, how it can be used to verify the applicability of the Sauerbrey relationship and their interaction with the viscoelasticity and in the biological sensing.

 


Download Full Technology Note

You can read and download the full Technology Note in pdf file from this link or download it from our Technology Web Page where you can find this and the rest of our Technology and Application Notes.

 

 

manufacturing

AWSensors cooperation with UPVfab

AWSensors, one of the first spin-off companies to emerge from the Polytechnic University of Valencia (UPV), sees a space for cooperation with UPVfab. Located at the Polytechnic City of Innovation (UPV’s Science Park), UPVfab is a breakthrough project, counting with a ~500 m2 clean room space and equipped with high-tech instruments, with applications in the areas of research in Photonics, Electronics and Chemistry.

In this initiative participate five research institutes located at the UPV: the Institute of Telecommunications and Multimedia Applications (iTEAM), the Instituto de Tecnología Quimica (ITQ-CSIC), the Instituto de Instrumentación para Imagen Molecular (I3M, UPV-CSIC), el Center for Research and Innovation in Bioengineering, and the Interuniversity Research Institute for Molecular Recognition and Technological Development (IDM) (IDM, UPV-UV).

However, this new project not only seeks to support academic research, but to strengthen its cooperation with industry partners as well. Prof. Antonio Arnau, full professor at UPV’s Department of Electronic Engineering and founder of AWSensors, acknowledges the importance of having these kind of installations that would make possible to validate new developments based on AWSensors’ proprietary array Quartz Crystal Microbalance with Dissipation (QCMD) biosensor technology. The company is already testing this technology in Healthcare applications, as an instrument for precision medicine through fast and simple diagnosis of cancer mutations for a personalized treatment and monitoring of its evolution.

AWSensors is always interested in productive public and private partnerships that help us further the developments in QCMD devices, sensors, and applications of interest.

 

Source: https://valenciaplaza.com/upv-crea-sala-limpia-investigar-fotonica-electronica-quimica

QCMD-webinar

QCM-D Webinar

May 24th 2021: AWSensors is pleased to invite you to participate in a QCM-D Scientific Webinar its Distributor Technex, in the BENELUX area, and AWSensors are orginizing. The scientific talk is entitled “Studying Soft Interfaces with Shear Waves: Principles and Applications of the Quartz Crystal Microbalance (QCM-D)” and  will be given by Prof. Diethelm Johannsmann.

 

QCMD Webinar

QCM-D Webinar details

The webinar will take place on Thursday, June 10th, 2021 from 15:00 to 16:30 hrs and it will be free of charge.

To join us, please register on this link: http://eepurl.com/gDLYUD and we will send you the Webinar details.

Speaker’s Short Biography

Diethelm Johannsmann is Professor of Physical Chemistry and the director of the Physical Chemistry Institute at Clausthal University of Technology, Germany. He has made fundamental contributions to the subject of QCM(D), reflected in more than 150 articles, several book chapters, and books. His model is widely used in the analysis of QCM-D data, and he developed free software for QCM-D data analysis and modelling.  You can find more information about his work on his website, https://www.pc.tu-clausthal.de/en/research/johannsmann-group/prof-dr-diethelm-johannsmann/.

 

QCMD Immunosensor

New QCMD Immunosensor Application Note

May 20th 2021: AWSensors is pleased to invite you to take a look to its new Immunosensor Application Note entitled “QCMD immunosensor for small molecule analytes“.

Summary of the Note

A QCMD-based immunoassay for label-free analysis of small molecule concentration in industrial samples was developed using an AWS QCMD system with surface-modified 5 MHz fundamental frequency QCMD sensors. Accuracy and precision of the immunoassay is evaluated with respect to the industry-standard HPLC reference.

QCMD Immunosensor

Introduction

Accurate, rapid, and cost-effective quantification of small molecule analytes is a pressing problem in various industrial (food, agriculture, environmental protection) and health-related fields. Existing approaches include enzyme-linked immunosorbent assays (ELISAs) and various types of chromatography (e.g., High Performance Liquid Chromatography, HPLC). These approaches require trained personal and centralized laboratories, and their deployment in the field is difficult or impossible.

Quartz Crystal Microbalance with Dissipation, or QCMD, is emerging as a promising technology for the development of fast, portable, automated, and cost-effective immunosensors. Here, we demonstrate a competitive small molecule immunoassay based on the AWSensors QCMD technology.


Download Full Application Note

You can download the full Application Note in pdf file from this link or download it from our Applications Web Page where you can find this and the rest of our Application Notes.

Other references

X4 QCMD System

X4 Launching

January 19th 2021: X4 launching

AWSensors is pleased to announce the launching of its new Advanced Multichannel QCMD system, the X4 Instrument, which allows the users to boost their productivity.

X4 Instrument


Visit X4 launching Landing-page

Learn more about this new instrument in its landing page.

QCMD in Lipid Research

QCMD in Lipid Research Tech Note

October 15th 2020: AWSensors is pleased to invite you to take a look to its Technology Note entitled “QCMD in Lipid Research”.

Summary of the Note

QCMD is a label-free surface-analytical technique based on a quartz resonator excited to oscillate at its resonance frequency on one or more overtones. Resonators can have various coatings: gold (Au), silica (SiO2), titania (TiO2), etc. It works in aqueous media or organic solvents and is therefore widely used for studying solid/liquid interfaces. At each overtone, QCMD measures changes in the resonance frequency and energy dissipation due to the processes occurring at the resonator surface. Examples of such processes include formation of a film or changes in the geometrical or physical properties of the film.

The key feature that makes QCMD useful in lipid research is its ability to distinguish between different geometries and topologies of lipidic assemblies at interfaces, for example, homogenous solid-supported bilayers or monolayers vs. adsorbed liposomes or other structures (such as cubosomes) without relying on fluorescent or deuterated labels but by relying on the combination of the frequency and dissipation.

QCMD in Lipid Research

Introduction

Lipid-related QCMD work can be grouped into several topics, with a total of more than a thousand publications:
• Studies focusing on the interactions between lipids and surfaces.
• Studies focusing on the properties of the lipids, such as their phase behavior, adsorbed liposome deformation, etc.
• Studies examining interactions between lipids and membrane-binding proteins, peptides or viruses. Particularly interesting is that QCMD offers a way to study clustering of membrane-bound proteins.
• Studies focusing on the interactions of lipids with polymers or with nanoparticles.

 

Continue reading by downloading the full Technology Note (below) …


Download Full Technology Note

You can download the full Note in pdf file from this link or download it from our Applications Web Page where you can find this and the rest of our Application and Technology Notes.

Tracking Recovery

Tracking Recovery Technology Note

September 15th 2020: AWSensors is pleased to invite you to take a look to its new Technology Note entitled “Tracking Recovery Technology Note”.

Summary of the Note

Use of the AWSensors X1 Instrument Tracking Recover feature to monitor overtones frequency and dissipation shifts of air-to-liquid medium exchanges onto 5 MHz QCM sensors.

Tracking Recovery

Introduction

The Tracking Recovery feature included in AWSensors X1 platform allows the user to monitor large and fast frequency shifts in QCM (Quartz Crystal Microbalance) admittance spectrum. These sudden modifications in the sensor response are common is some applications where dramatical changes in the viscoelastic properties of the sensor surrounding medium take place.

This technical note illustrates the utility of tracking recovery feature to characterize an air-to-liquid medium exchange. According to Kanazawa and Gordon theory predictions [1], a complex frequency shift is expected in the sensor electromechanical response when the semi-infinite medium placed over the QCM’s top electrode is replaced by other semi-infinite medium. This shift will depend on the viscosity and density properties of the final medium. Following, Kanazawa-Gordon equation is presented for both the frequency (Eq. 1) and the half-bandwidth (Eq. 2) shifts.

Continue reading by downloading the full Technology Note (below) …


Download Full Technology Note

You can download the full Note in pdf file from this link or download it from our Technology Web Page where you can find this and the rest of our Application and Technology Notes.

Carbaryl Biosensor based on antibody detection

Biosensor Application Note

July 3rd 2020: AWSensors is pleased to invite you to take a look to the Biosensor Application Note entitled “Acoustic Biosensor“.

Summary of the Note

An immunosensor application for determination of carbaryl pesticide was developed by using AWS A20 research platform and AWS F20 Fluidic System. Carbaryl was chosen as the model analyte. Two kinds of acoustic sensors were employed: AWS HFF-QCM sensors (50 MHz and 100 MHz) and Love-SAW sensors with appropriate cells. The AWS A20 platform allowed monitoring phase-shift changes at constant frequency as a function of the sensor surface mass changes.

Biosensor

Introduction

Sensor functionalization: Carbaryl hapten conjugate was covalently immobilized by means of Self Assembled Monolayer (SAM).

Immunoassay format: The chosen competitive immunoassay was a binding-inhibition test based on conjugate-coated format. Carbaryl analyte competes against the immobilized hapten-conjugate for Monoclonal Antibodies.

Carbaryl detection: Samples were injected onto the sensors’ surfaces. AWS software allowed controlling sample injection and fluidics. Furthermore, the employed platform allowed performing the measurements at a constant temperature of 25°C ± 0.05°C.

Since analyte inhibits antibody binding to its respective immobilized conjugates, increasing concentrations of analyte are detected by a change in the increment of the phase-shift of the sensor. The following figures present a representative assay cycle selected from a continuous monitoring in a carbaryl determination, for 100 MHz HFF QCM and 120MHz Love Wave Sensors.

Continue reading by downloading the full Application Note (below) …


Download Full Application Note

You can download the full Application Note in pdf file from this link or download it from our Applications Web Page where you can find this and the rest of our Application and Technology Notes.

QCMD

QCMD New Technology Note