Tag Archive for: QCMD

Publication on AWSensors technology

Fouling of Reverse Osmosis Membrane with Effluent Organic Matter: Componential Role of Hydrophobicity

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Authors: Noa Stein, Revital Sharon-Gojman, Meagan S. Mauter, Roy Bernstein and Moshe Herzberg

Journal: ACS ES&T Water (2023)

 

Abstract

Organic matter dissolved in tertiary effluents (effluent organic matter, EfOM) is the predominant organic membrane foulant in tertiary wastewater reverse osmosis (RO) desalination, ultimately causing biofouling. The interrelated effects of EfOM fractions of different hydrophobicity and polarity on membrane performance were studied by (i) examining each fraction’s overall effect on membrane permeability; (ii) analyzing the intrinsic hydraulic resistance induced by each fraction; (iii) studying their adsorption on the active layer of an RO membrane using a quartz crystal microbalance with dissipation monitoring (QCM-D); (iv) assessing their “dry” molecular mass when adsorbed on polyamide using localized surface plasmon resonance (LSPR) sensing; (v) analyzing their hydrodynamic radii by dynamic light scattering (DLS); and (vi) characterization using excitation–emission matrix (EEM) analysis and parallel-factor (PARAFAC) modeling. Hydrophobic and transphilic neutral fractions (containing ∼12.5% total organic carbon) have the greatest effect on membrane flux reduction and the highest hydraulic resistance and adhere most strongly to polyamide surfaces, resulting in the highest adsorbed “dry” mass. Therefore, in terms of their effect on RO permeate flux reduction, these fractions are the most detrimental in the EfOM mix. EEM analysis and associated PARAFAC modeling indicate that the main components causing this effect are mixtures of protein-like compounds, together with humic-like substances. Novel LSPR-based analysis elucidated the role of the fractions most detrimental to membrane permeability through measurement of dry mass surface concentration on a polyamide mimetic sensor. This study provides valuable insights into the roles of different EfOM fractions in RO membrane fouling and enhances our understanding of fouling during tertiary wastewater desalination.

Fouling of Reverse Osmosis Membrane with Effluent Organic Matter: Componential Role of Hydrophobicity

 

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Tuning the water interactions of cellulose nanofibril hydrogels using willow bark extract

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Authors: Ngoc Huynh, Juan José Valle-Delgado, Wenwen Fang, Suvi Arola, Monika Österberg

Journal: Carbohydrate Polymers (2023)

 

Abstract

Cellulose nanofibrils (CNFs) are increasingly used as precursors for foams, films and composites, where water interactions are of great importance. In this study, we used willow bark extract (WBE), an underrated natural source of bioactive phenolic compounds, as a plant-based modifier for CNF hydrogels, without compromising their mechanical properties. We found that the introduction of WBE into both native, mechanically fibrillated CNFs and TEMPO-oxidized CNFs increased considerably the storage modulus of the hydrogels and reduced their swelling ratio in water up to 5–7 times. A detailed chemical analysis revealed that WBE is composed of several phenolic compounds in addition to potassium salts. Whereas the salt ions reduced the repulsion between fibrils and created denser CNF networks, the phenolic compounds – which adsorbed readily on the cellulose surfaces – played an important role in assisting the flowability of the hydrogels at high shear strains by reducing the flocculation tendency, often observed in pure and salt-containing CNFs, and contributed to the structural integrity of the CNF network in aqueous environment. Surprisingly, the willow bark extract exhibited hemolysis activity, which highlights the importance of more thorough investigations of biocompatibility of natural materials. WBE shows great potential for managing the water interactions of CNF-based products.

 

Cellulose nanofibrils (CNFs) are increasingly used as precursors for foams, films and composites, where water interactions are of great importance. In this study, we used willow bark extract (WBE), an underrated natural source of bioactive phenolic compounds, as a plant-based modifier for CNF hydrogels, without compromising their mechanical properties. We found that the introduction of WBE into both native, mechanically fibrillated CNFs and TEMPO-oxidized CNFs increased considerably the storage modulus of the hydrogels and reduced their swelling ratio in water up to 5–7 times. A detailed chemical analysis revealed that WBE is composed of several phenolic compounds in addition to potassium salts. Whereas the salt ions reduced the repulsion between fibrils and created denser CNF networks, the phenolic compounds - which adsorbed readily on the cellulose surfaces - played an important role in assisting the flowability of the hydrogels at high shear strains by reducing the flocculation tendency, often observed in pure and salt-containing CNFs, and contributed to the structural integrity of the CNF network in aqueous environment. Surprisingly, the willow bark extract exhibited hemolysis activity, which highlights the importance of more thorough investigations of biocompatibility of natural materials. WBE shows great potential for managing the water interactions of CNF-based products.

 

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Publication on AWSensors technology

Trace Water Effects on Crystalline 1-Ethyl-3-methylimidazolium Acetate

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Authors: Ashlee Aiello, John R. Hoffman, Anthony P. Kotula, Lucas Q. Flagg, Ruipeng Li, and Jeremiah W. Woodcock

Journal: J. Phys. Chem. B (2023)

 

Abstract

Spontaneous room-temperature crystallization of 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) was observed upon removal of trace water. Sample purity was confirmed using analytical nuclear magnetic resonance spectroscopy to ensure that trace water or other contaminants did not produce this observation. Raman spectroscopy and simultaneous quartz crystal microbalance/infrared spectroscopy measurements were used to study molecular reorganization during crystallization and decrystallization using trace water in the form of atmospheric moisture. These experimental results were supplemented with density functional theory calculations that indicate imidazolium cation ring stacking and side chain clustering with an exclusive arrangement of the acetate anion in the cation ring plane upon water removal. Crystal structure formation was confirmed using two-dimensional wide-angle X-ray scattering. This natural crystallization is attributed to the removal of trace water over extended periods of time and calls attention to the molecular-level role of water in the structure of hygroscopic ionic liquid systems.

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Multispecies biofilms on reverse osmosis membrane dictate the function and characteristics of the bacterial communities rather than their structure

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Authors: Noya Ran, Gil Sorek, Noa Stein, Revital Sharon-Gojman, Moshe Herzberg, and Osnat Gillor

Journal: Environmental Research (2023)

 

Abstract

The main reason for the deterioration of membrane operation during water purification processes is biofouling, which has therefore been extensively studied. Biofouling was shown to reduce membrane performance reflected by permeate flux decline, reduced selectivity, membrane biodegradation, and consequently, an increase in energy consumption. Studies of biofouling focused on the identification of the assembled microbial communities, the excretion of extracellular polymeric substances (EPS), and their combined role in reduced membrane performance and lifetime. However, the link between the structure and function of biofouling communities has not been elucidated to date. Here, we provide a novel insight, suggesting that bacterial functions rather than composition control biofouling traits on reverse osmosis (RO) membranes. We studied the potential activity of RO biofilms at metatranscriptome resolution, accompanied by the morphology and function of the biofouling layer over time, including microscopy and EPS composition, adhesion, and viscoelastic properties. To that end, we cultivated natural multispecies biofilms in RO membranes under treated wastewater flow and extracted RNA to study their taxonomies and gene expression profiles. Concomitantly, the biofilm structure was visualized using both scanning electron microscopy and laser scanning confocal microscopy. We also used quartz crystal microbalance with dissipation to characterize the affinity of EPS to membrane-mimetic sensors and evaluated the viscoelasticity of the Ex-Situ EPS layer formed on the sensor. Our results showed that different active bacterial taxa across five taxonomic classes were assembled on the RO membrane, while the composition shifted between 48 and 96 h. However, regardless of the composition, the maturation of the biofilm resulted in the expression of similar gene families tightly associated with the temporal kinetics of the EPS composition, adhesion, and viscoelasticity. Our findings highlight the temporal selection of specific microbial functions rather than composition, featuring the adhesion kinetics and viscoelastic properties of the RO biofilm.

 

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Effect of Noise on Determining Ultrathin-Film Parameters from QCM-D Data with the Viscoelastic Model

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Authors: Diethelm Johannsmann, Arne Langhoff, Christian Leppin, Ilya Reviakine, and Anna M. C. Maan

Journal: Sensors (2023)

 

Abstract

Quartz crystal microbalance with dissipation monitoring (QCMD) is a well-established technique for studying soft films. It can provide gravimetric as well as nongravimetric information about a film, such as its thickness and mechanical properties. The interpretation of sets of overtone-normalized frequency shifts, Δf/n, and overtone-normalized shifts in half-bandwidth, ΔΓ/n, provided by QCMD relies on a model that, in general, contains five independent parameters that are needed to describe film thickness and frequency-dependent viscoelastic properties. Here, we examine how noise inherent in experimental data affects the determination of these parameters. There are certain conditions where noise prevents the reliable determination of film thickness and the loss tangent. On the other hand, we show that there are conditions where it is possible to determine all five parameters. We relate these conditions to the mathematical properties of the model in terms of simple conceptual diagrams that can help users understand the model’s behavior. Finally, we present new open source software for QCMD data analysis written in Python, PyQTM.

 

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Nanoporous Metal–Organic Framework Thin Films Prepared Directly from Gaseous Precursors by Atomic and Molecular Layer Deposition: Implications for Microelectronics

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Authors: Jenna Multia, Dmitry E. Kravchenko, Víctor Rubio-Giménez, Anish Philip, Rob Ameloot, and Maarit Karppinen

Journal: ACS Appl. Nano Mater. (2023)

 

Abstract

Atomic/molecular layer deposition (ALD/MLD) allows for the direct gas-phase synthesis of crystalline metal–organic framework (MOF) thin films. Here, we show for the first time using krypton and methanol physisorption measurements that ALD/MLD-fabricated copper 1,4-benzenedicarboxylate (Cu-BDC) ultrathin films possess accessible porosity matching that of the corresponding bulk MOF.

 

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Acoustic Array Biochip Combined with Allele-Specific PCR for Multiple Cancer Mutation Analysis in Tissue and Liquid Biopsy

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Authors: Nikoletta Naoumi, Kleita Michaelidou, George Papadakis, Agapi E. Simaiaki, Román Fernández, Maria Calero, Antonio Arnau, Achilleas Tsortos, Sofia Agelaki, and Electra Gizeli

Journal: ACS Sens. (2022)

Abstract

Regular screening of point mutations is of importance to cancer management and treatment selection. Although techniques like next-generation sequencing and digital polymerase chain reaction (PCR) are available, these are lacking in speed, simplicity, and cost-effectiveness. The development of alternative methods that can detect the extremely low concentrations of the target mutation in a fast and cost-effective way presents an analytical and technological challenge. Here, an approach is presented where for the first time an allele-specific PCR (AS-PCR) is combined with a newly developed high fundamental frequency quartz crystal microbalance array as biosensor for the amplification and detection, respectively, of cancer point mutations. Increased sensitivity, compared to fluorescence detection of the AS-PCR amplicons, is achieved through energy dissipation measurement of acoustically “lossy” liposomes binding to surface-anchored dsDNA targets. The method, applied to the screening of BRAF V600E and KRAS G12D mutations in spiked-in samples, was shown to be able to detect 1 mutant copy of genomic DNA in an excess of 104 wild-type molecules, that is, with a mutant allele frequency (MAF) of 0.01%. Moreover, validation of tissue and plasma samples obtained from melanoma, colorectal, and lung cancer patients showed excellent agreement with Sanger sequencing and ddPCR; remarkably, the efficiency of this AS-PCR/acoustic methodology to detect mutations in real samples was demonstrated to be below 1% MAF. The combined high sensitivity and technology-readiness level of the methodology, together with the ability for multiple sample analysis (24 array biochip), cost-effectiveness, and compatibility with routine workflow, make this approach a promising tool for implementation in clinical oncology labs for tissue and liquid biopsy.

 

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V. cholerae MakA is a cholesterol-binding pore-forming toxin that induces non-canonical autophagy

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Authors: Xiaotong Jia, Anastasia Knyazeva, Yu Zhang, Sergio Castro-Gonzalez, Shuhei Nakamura, Lars-Anders Carlson, Tamotsu Yoshimori, Dale P. Corkery, Yao-Wen Wu

Journal: J Cell Biol (2022)

 

Abstract

Pore-forming toxins (PFTs) are important virulence factors produced by many pathogenic bacteria. Here, we show that the Vibrio cholerae toxin MakA is a novel cholesterol-binding PFT that induces non-canonical autophagy in a pH-dependent manner. MakA specifically binds to cholesterol on the membrane at pH < 7. Cholesterol-binding leads to oligomerization of MakA on the membrane and pore formation at pH 5.5. Unlike other cholesterol-dependent cytolysins (CDCs) which bind cholesterol through a conserved cholesterol-binding motif (Thr-Leu pair), MakA contains an Ile-Ile pair that is essential for MakA-cholesterol interaction. Following internalization, endosomal acidification triggers MakA pore-assembly followed by ESCRT-mediated membrane repair and V-ATPase-dependent unconventional LC3 lipidation on the damaged endolysosomal membranes. These findings characterize a new cholesterol-binding toxin that forms pores in a pH-dependent manner and reveals the molecular mechanism of host autophagy manipulation.

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Aerosol Jet Printing of the Ultramicroporous Calcium Squarate Metal–Organic Framework

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Authors: Dmitry E. Kravchenko, Aleksander Matavž, Víctor Rubio-Giménez, Hanne Vanduffel, Margot Verstreken, Rob Ameloot

Journal: Chem. Mater. (2022)

 

Abstract

Efficient methods to deposit thin layers of metal–organic frameworks (MOFs) are needed to integrate these microporous materials into microelectronics, sensing devices, and membranes. Herein, we report for the first time the direct aerosol jet printing of a MOF material. The ultramicroporous MOF [Ca(C4O4) (H2O)] (UTSA-280) was deposited from an aqueous precursor solution. In addition to blanket coatings, aerosol jet printing provides direct access to patterned coatings with a resolution of 100 μm via a digital, maskless approach. Moreover, by enabling spatial control over the layer thickness via the number of passes of the nozzle, this direct-write approach presents a more accessible alternative to advanced patterning techniques such as grayscale lithography.

 

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AWSensors participa en el programa Industria 4.0

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ADVANCED WAVE SENSORS, S.L. (AWSensors) ha sido beneficiario del Fondo Europeo de Desarrollo Regional cuyo objetivo es mejorar el uso y la calidad de las tecnologías de la información y de las comunicaciones y el acceso a las mismas y gracias al que ha desarrollado los proyectos de “Gestión integral (ERP)” y “Sistemas de diseño asistido por computador e impresión 3D para prototipo rápido“, para la mejora de competitividad y productividad de la empresa. (30/06/2022). Para ello ha contado con el apoyo del programa Industria 4.0 de la Cámara de Comercio de Valencia. Una manera de hacer Europa


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